RESUMO
A soil streptomycete (Streptomyces sp. A11) degraded collagen isolated from bovine Achilles tendon, calf skin, human placenta, carp swim bladder and rat tail tendon and released appreciable quantities of hydroxyproline. It also degraded hide powder and vegetable tanned leather. The organism was taxonomically characterized, compared with allied species, identified and designated as Streptomyces wartii.
Assuntos
Animais , Bovinos , Colágeno/isolamento & purificação , Colagenases/biossíntese , Feminino , Humanos , Microscopia Eletrônica de Varredura , Gravidez , Ratos , Microbiologia do Solo , Streptomyces/classificaçãoAssuntos
Animais , Proteínas de Bactérias/isolamento & purificação , Biodegradação Ambiental , Galinhas , Endopeptidases/isolamento & purificação , Plumas/efeitos dos fármacos , Cabras , Cabelo/efeitos dos fármacos , Humanos , Hidrólise , Microbiologia Industrial , Proteínas de Insetos , Queratinas/efeitos dos fármacos , Proteínas/efeitos dos fármacos , Seda , Microbiologia do Solo , Streptomyces/enzimologia , Lã/efeitos dos fármacosRESUMO
Keratinase produced from Streptomyces Sp.A11 decomposed human hair, chicken feather, wool, silk and pure keratin extracted from human epidermis. Purification of the enzyme by DEAE-cellulose column chromatography resulted in 7.5-fold increase in activity relative to the activity of the culture filtrate. The enzyme was inducible, extracellular, homogeneous with a molecular weight of 49,000. The enzyme activity was inhibited by reduced glutathione, phenylmethyl sulphonyl fluoride and 2-mercaptoethanol.